ABSTRACT
Objective To investigate the effects of CCR10 expression on proliferation,migration and invasion of NSCLC cells.Methods CCR10 siRNA was conducted into humanlung adenocarcinoma cellA549 (A549) to interfere with the normal expression of CCR10,and then to study how dose CCR10 have effects on cell proliferation,migrationand invasion in NSCLC.Results The results of MTT showed that the proliferation rate of A549 conducted withCCR10 siRNA was significantly decreased compared with Negative Control (NC) and Mock(P ＜ 0.05).The proliferation rate of A549 in NC compared with Mock has nostatistical significance (P ＞ 0.05).The results of Transwell cell migration assay showed that the migration rate of A549 conducted with CCR10 siRNA was significantly decreased compared with NC and Mock(P ＜ 0.05).The proliferation rate of A549 in NC compared with Mock has nostatistical significance (P ＞0.05).The results of Transwell cell invasion assay showed that the invasion rate ofA549 conducted with CCR10 siRNA was significantly decreased compared with NC and Mock(P ＜ 0.05).The invasion rate of A549 in NC compared with Mock has nostatistical significance (P ＞0.05).Conclusion CCR10 may contribute to cell proliferation,migration and invasion in NSCLC,and associated with the occurrence and development of NSCLC.
ABSTRACT
Objective To investigate the expression of chemokine CCL27,CCL28 and their receptor CCR10 in mouse periph-eral blood in the acute phase of epilepsy.Methods The peripheral blood of acute epileptic mice at different time points(10 min,30 min,1 h,2 h)was collected,real-time PCR was used to detect the mRNA expression level of CCL27 and CCL28.The heparin anti-coagulation peripheral blood at the same time points(10 min,30 min,1 h,2 h)of normal and acute phase of epileptic mice were col-lected and flow cytometry was used to investigate the expression of CCR10 in peripheral blood lymphocyte.Results The mRNA expression level of CCL27,CCL28 in peripheral blood and the expression of CCR10 in lymphocytes were found significantly in-creased at 2 h in epileptic mice than those of normal(P <0.01).Conclusion The immune function disorder occured in peripheral blood in early epilepsic pathological process and might be associated with the subsequent inflammatory reaction and neuron apoptosis.